Recombinant Human UCHL1 Protein(His tag) (HE0191UL)

Recombinant Human UCHL1 Protein (P09936) (Gln 2-Ala 223) with a polyhistide tag at the N-terminus was expressed in E. coli.

Datasheet

Size	Price	Qty
20ug	$429.00

Order Online Bulk Inquiry

PRODUCT INFORMATION

Cat.No.

HE0191UL

Synonyms

UCHL1; PARK5; PGP9.5; Uch L1; PGP95; Uch-L1; PGP 9.5

Accession

UCHL1

Predicted N Terminal

Met

Form

Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by the hydrolysis of UbiquitinAMC. The specific activity is>140 pmoles/min/μg.

Molecular Mass

Recombinant Human UCHL1 consisting of 229 amino acids and migRates as an 25.6 kDa band in SDS-PAGE under reducing conditions as predicted.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 92 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Tag

His

Species

Human

Source

E. coli

BACKGROUND

Background