Recombinant Human IL2RB Protein(DDDDK tag) (HH0139IL)

Recombinant Human IL2RB Protein (P14784) (Met 1-Asp 239) with five amino acids (DDDDK) at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0139IL

Synonyms

IL2RB; CD122; p75; IL-2RB; IL15RB; P70-75;

Accession

IL2RB

Predicted N Terminal

Ala 27

Form

Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human IL2Rb at 10 μg/ml (100 μl/well) can bind biotinylated human IL2, The EC50 of biotinylated human IL2 is 0.1-0.24 μg/mL. Measured by its ability to inhibit IL15-dependent proliferation

Molecular Mass

Recombinant Human IL2 Rβ consists of 219 amino acids and has a predicted molecular mass of 25.2 kDa. As a result of glycosylation, the rh IL2 Rβ migRates as an approximately 40 kDa protein in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Tag

DDDDK

Species

Human

Source

HEK293

BACKGROUND

Background

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.