Recombinant Human CD274 Protein(Fc tag) (HH0085CL)

Recombinant Human CD274 Protein (Q9NZQ7) (Met 1-Thr 239) with the C-terminal Fc region of human IgG1 was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0085CL

Synonyms

CD274; B7 H; B7 H1; B7H1; PD L1; PDL1; B7-H; PD-L1; PDCD1L1; PDCD1LG1;

Accession

CD274

Predicted N Terminal

Phe 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human PD-1 at 10 μg/ml (100 μl/well) can bind recombinant human B7-H1 / PD-L1 / Fc chimera with a linear range of 0.02-0.4 μg/ml.

Molecular Mass

RecombinantHuman B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the rh B7-H1/Fc monomer migRates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

Tag

Species

Human

Source

HEK293

BACKGROUND

Background