Recombinant Human BMP2 Protein (HE0063BL)

Cat.No.

HE0063BL

Synonyms

BMP2; BMP2A; BMP-2A; BDA2;

Accession

BMP2

Form

Lyophilized from sterile 10mM sodium citrate, pH3.5.

Bio-activity

BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less than 50 ng/ml, corresponding to a specific activity of 2.0 x 10^4 IU/mg.

Molecular Mass

Recombinant Human BMP2 Protein is a homodimeric, non-glycosylated, Polypeptide chain and has a molecular mass of approximately 26.018 kDa.

Endotoxin

< 0.01 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Dissolve with double distilled water.

Warning

For research use only!

Background

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

Species

Human

Source

E. coli

Background

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).