Protein Mutagenesis
Mutagenesis is an important molecular biology technique in the laboratory that facilitates life science research by making specific and intentional changes to the DNA sequence of genes and any products. It can be used to study the structure and biological activity of DNA, RNA, and protein, as well as DNA-protein interactions, enzyme active sites, and novel proteins.
Most mutagenesis is carried out based on PCR. PCR can be performed in the laboratory, however, various tests and optimizations are required during the experiment, and personnel proficient in PCR and primer design are required. Therefore, in order to help customers save time spent in streamlined operations, Profacgen provides fast and cost-effective services to obtain 100% accurate mutant DNA clones, customers no longer need to buy various kits and spend time in the laboratory for PCR mutagenesis, cloning and sequencing. We can manufacture any gene template and unlimited number of mutations, as well as comprehensive upstream and downstream services.
Our Services
Precise mutagenesis of target proteins is very useful in the study of protein-protein interactions, protein structure, biological active sites, etc. Utilizing state-of-the-art DNA synthesis technology, Profacgen provides efficient protein mutagenesis services, as well as comprehensive upstream and downstream services, including template DNA sequencing, de novo gene synthesis, expression vector construction, and protein expression and purification. We can also create protein truncations according to your specific requirements.
Mutagenesis services
- Site-directed mutagenesis: Single or multiple mutations (substitutions, insertions, or deletions) can be introduced into your protein of interest with high precision and efficiency, without creating unwanted mutations.
- Random mutagenesis: Mutations can be introduced into genes using error-prone PCR, or you can decide exactly which parts of your genes are affected.
Highlights
- High accuracy confirmed by full sequencing
- Mutations can be introduced at any site
- Mutations on Large DNA Constructs
- Cost-effective
- Short turnaround time
Profacgen has accumulated lots of experience in protein mutagenesis. Our professional technical team can provide customers with high-quality site-directed and random mutagenesis and many related featured services you may be interested in. Our competitive prices and extensive expertise have earned us the trust of our collaborators. Contact us to find out how Profacgen could be of assistance.